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KMID : 0545120040140040877
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 4 p.877 ~ p.880
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Chitooligosaccharides in Korean Commercial Salt-Fermented Shrimps, Determined by Enzyme-Linked Immunosorbent Assay
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Shim YY
Shon DH/Chee KM
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Abstract
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In this study we determined the content chitooligosaccharides (COS) in Korean commercial salt-fermented shrimps by competitive direct enzyme-linked immunosorbent assays (cdELISAs) using anti-COS mixture (COSM) antibody and COSM horseradish peroxidase (HRP) conjugate. When COS6 was spiked into salt-fermented shrimps at the level 10- 300 μg/g the average recovery was 120±19% (mean S.D.). The COS contents of the 92 samples of Korean commercial salt-fermented shrimps collected during February 2000 and August 2002 were 36.3±20.7 μg COS6 equivalent/ (expressed as “μg/g” hereafter). Among the samples the COS contents of yuk-jeot (40.3±22.5 μg/g n=27) and buksaewoojeot (40.2±21.6 μg/g n=5) were higher than the others. The COS contents of salt-fermented shrimps produced at Gwangcheon (47.1±20.7 μg/g n=18) and Gomso (44.1 21.8 μg/g n=6) areas were higher than those produced at the other areas. This is the first report to determine COS of saltfermented shrimps by cdELISA.
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